Method for screening genomic dna fragments

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/09 (2006.01) A01H 5/00 (2006.01) C12N 5/14 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2547514

It is intended to provide a method of selecting a genomic DNA fragment to be transferred into a plant for making an agriculturally advantageous improvement in the plant. Namely, a method comprising: (1) the step of preparing genomic DNA from a plant and constructing a genomic DNA library with the use of a cloning vector; (2) transferring individual genomic fragments contained in a plural number of genomic clones constituting the genomic DNA library into plants to give transformant plants; (3) culturing the transformant plants or offspring plants thereof and selecting a plant having an agriculturally advantageous mutation in the phenotype; and (4) selecting, as a desired genomic DNA fragment, a genomic DNA fragment having been transferred in the step (2) into the plant selected in the step (3).

L'invention concerne un procédé de sélection de fragment d'ADN génomique à transférer dans une plante, donnant une amélioration importante du point de vue agricole, selon les étapes suivantes : (1) sélection d'ADN génomique dans la plante et élaboration d'une librairie d'ADN génomique par le biais d'un vecteur de clonage ; (2) transfert, sur des plantes, de fragments génomiques individuels contenus dans une pluralité de clones génomiques constituant la librairie d'ADN génomique pour donner des plantes qui sont des transformants ; (3) mise en culture des transformants en question ou des plantes issues de ces transformants et sélection d'une plante à mutation avantageuse du point de vue agricole dans le phénotype ; et (4) sélection, comme fragment d'ADN génomique souhaité, de fragment d'ADN génomique ayant été transféré à l'étape (2) dans la plante sélectionnée à l'étape (3).

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