Method for secreting thrombopoietin polypeptides

Details Method for secreting thrombopoietin polypeptides Method for secreting thrombopoietin polypeptides

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) C07K 14/52 (2006.01) C12N 1/19 (2006.01) C12N 5/10 (2006.01) C12N 9/72 (2006.01) C12N 15/19 (2006.01) C12N 15/58 (2006.01) C12P 21/06 (2006.01)

Patent

CA 2206399

DNA constructs useful in the production of thrombopoietin are disclosed. In general, the DNA constructs comprise a first DNA segment encoding a fusion of an amino-terminal secretory peptide joined to a thrombopoietin polypeptide and one or more additional DNA segments that provide for the transcription of the first segment. The secretory peptide is a native mammalian t-PA secretory peptide or may be modified to enhance proteolytic cleavage of the fusion. Also disclosed are cultured eukaryotic cells containing these DNA constructs and methods for producing thrombopoietin polypeptides through the use of the DNA constructs and cultured eukaryotic cells.

Produits d'ADN de recombinaison utiles dans la production de thrombopoïétine. En général, lesdits produits d'ADN de recombinaison comportent un premier segment d'ADN codant une fusion d'un peptide sécréteur à terminaison amino joint à un polypeptide de thrombopoïétine et un ou plusieurs segments d'ADN supplémentaires qui permettent la transcription du premier segment. Le peptide sécréteur est un peptide t-PA mammalien natif, ou un peptide qui peut être modifié pour favoriser le clivage protéoloytique de la fusion. Des cellules eucaryotes contenant ces produits d'ADN de recombinaison et des procédés destinés à la production de polypeptides de thrombopoïétine en utilisant lesdits produits d'ADN de recombinaison et lesdites cellules eucaryotes cultivées sont également décrits.

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