Method for selecting and producing vaccine components and...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/31 (2006.01) A61K 39/09 (2006.01) A61K 39/40 (2006.01) A61P 31/04 (2006.01) A61P 37/04 (2006.01) C07K 14/315 (2006.01) C07K 16/12 (2006.01) C12Q 1/00 (2006.01)

Patent

CA 2525766

The invention relates to the field of biology, more specifically to the field of immunology and microbiology. The invention further relates to the field of vaccines against microbial infections and especially bacterial vaccines, in particular to pneumococcal vaccines. More in particular, the invention relates to means and methods to identify, select and isolate a vaccine component for passive and/or active immunisation against a microorganism that can be killed by opsonophagocytic cells. The invention relates to a method to identify an opsonophagocytosis inducing antigen as a vaccine component for immunisation against a microorganism. The invention describes three pneumococcal proteins SlrA, IgA1 proteinase, and PsaA, and their use as a vaccine component with or without PpmA. The invention also discloses the use of antibodies against said proteins for passive immunization and diagnosis.

L'invention concerne le domaine de la biologie et, plus spécifiquement, le domaine de l'immunologie et de la microbiologie. L'invention concerne en outre le domaine des vaccins contre des infections microbiennes, notamment des vaccins bactériens et, en particulier, des vaccins antipneumococciques. Plus particulièrement, l'invention concerne des moyens et des procédés permettant d'identifier, de sélectionner et d'isoler un composant de vaccin pour une immunisation passive et/ou active contre un micro-organisme qui peut être tué par des cellules opsonophagocytiques. L'invention concerne un procédé permettant d'identifier un antigène occasionnant une opsonophagocytose, en tant que composant d'un vaccin, en vue d'une immunisation contre un micro-organisme. L'invention décrit trois protéines pneumococciques S1rA, protéinase IgA1 et PsaA, et leur utilisation comme composant de vaccin avec, ou sans PpmA. Enfin, l'invention concerne l'utilisation d'anticorps contre lesdites protéines, en vue de l'immunisation passive et du diagnostic.

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