Method for selecting antibody expressing cells

Details Method for selecting antibody expressing cells Method for selecting antibody expressing cells

G - Physics

01

N

G01N 33/569 (2006.01)

Patent

CA 2454088

A method for selecting high level antibody producing cells, comprising the step of attaching protein A or protein G to a cell surface, thus capturing secreted, soluble antibody. Fluorescence staining of the captured antibody by conventional methods and subsequent FACS analysis allows to select low abundance high producer single cells.

Procédé pour sélectionner les cellules produisant des anticorps à haut niveau, qui consiste à attacher une protéine A ou une protéine G à une surface de cellule puis capturer un anticorps soluble sécrété. La coloration par fluorescence de l'anticorps capturé par des procédés traditionnels et l'analyse FACS subséquente permet de sélectionner les cellules uniques hautement productives à faible abondance.

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