Method for selecting genetically transformed cells

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 5/04 (2006.01) C12N 9/24 (2006.01)

Patent

CA 2466088

An environmental friendly and non toxic method for selection of transformed cells from a population consisting of transformed and non-trasnsformed cells. The method comprises the following steps a) introducing into a cell at least one desired nucleotide sequence and at least one selection-nucleotide sequence to obtain a genetically transformed cell, wherein the selection-nucleotide sequence comprises a region which codes for a protein involved in the metabolizing of trehalose; b) placing a population with transformed and non- transformed cells into contact with trehalose and/or derivative thereof; and c) selecting the transformed cells from the population on the basis of the capacity of the transformed cells to metabolize the trehalose and/or derivative.

L'invention concerne une méthode non toxique et respectueuse de l'environnement permettant de sélectionner des cellules transformées provenant d'une population constituée de cellules transformées et non transformées. Cette méthode consiste à a) introduire dans une cellule au moins une séquence de nucléotides souhaitée et au moins une séquence de nucléotides de sélection afin d'obtenir une cellule génétiquement transformée, la séquence de nucléotides de sélection comportant une région qui code une protéine impliquée dans la métabolisation de la tréhalose, b) à placer une population possédant des cellules transformées et non transformées en contact avec la tréhalose et/ou un dérivé correspondant, et c) à sélectionner les cellules transformées à partir de la population sur la base de la capacité des cellules transformées à métaboliser la tréhalose et/ou un dérivé correspondant.

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