Method for selecting hybridomas producing antibodies...

Details Method for selecting hybridomas producing antibodies... Method for selecting hybridomas producing antibodies...

C - Chemistry, Metallurgy

07

K

530/15.06, 195/1

C07K 16/44 (2006.01) G01N 33/577 (2006.01)

Patent

CA 1263324

Abstract The invention provides a method for selecting hybridomas which produce antibodies specific to domains of a cell surface antigen which is usually not accessible at the surface of intact cells. The method employs the screening of the hybridoma clones obtained for the production of antibodies specific against the cell surface antigen by use of immunoblotting analysis, namely by screening the clones against antigen immobilized on a solid substrate such as nitrocellulose. The invention also includes those hybridomas and monoclonal antibodies when produced according to the method.

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