Method for selecting improved vectors

Details Method for selecting improved vectors Method for selecting improved vectors

C - Chemistry, Metallurgy

12

N

C12N 15/86 (2006.01) A61K 48/00 (2006.01) C07K 14/15 (2006.01) C07K 14/155 (2006.01) C12N 5/06 (2006.01) C12N 5/10 (2006.01) C12N 7/00 (2006.01) C12N 15/10 (2006.01) C12N 15/867 (2006.01)

Patent

CA 2365564

A method is provided for selecting an improved retroviral genome having an improved packaging efficiency which method comprises: a) introducing one or more random mutations into a retroviral genome comprising a packaging signal; b) introducing the mutagenised retroviral genome into a host cell expressing viral polypeptides required for packaging the retroviral genome; c) determining whether retroviral packaging efficiency in the cell is improved as compared with a retroviral genome comprising a non-mutated packaging signal; d) selecting a viral genome which has improved packaging efficiency.

L'invention concerne un procédé permettant de sélectionner un génome rétroviral amélioré présentant une efficacité d'encapsidation accrue. Ce procédé comprend les étapes suivantes: a) on introduit une ou plusieurs mutations aléatoires dans un génome rétroviral comprenant un signal d'encapsidation; b) on introduit le génome rétroviral mutagénisé dans une cellule hôte exprimant les polypeptides viraux nécessaires à l'encapsidation du génome rétroviral; c) on détermine si l'efficacité de l'encapsidation rétrovirale dans la cellule est améliorée par comparaison à un génome rétroviral comprenant un signal d'encapsidation non mutant; d) on sélectionne un génome viral présentant une efficacité d'encapsidation améliorée.

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