Method for selection of insertion mutations

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) A01H 5/00 (2006.01)

Patent

CA 2303907

The present invention provides a highly-efficient method for the selection and identification of insertional mutants. In the technique, a non-selective amplification is used to isolate a plurality of insertion events from a population of individuals comprising insertion mutations. Specific insertion events can then be identified from the population by the use of gene specific probes or primers. Through the identification of mutants for a particular gene, data may be obtained regarding the function and phenotypic effects of that gene, and thereby, the gene can be employed in the creation of novel biotechnological products.

L'invention concerne un procédé hautement efficace permettant de sélectionner et d'identifier des mutants d'insertion, qui consiste à utiliser une amplification non sélective pour isoler une pluralité d'événements d'insertion, dans une population d'individus comportant des mutations par insertion. Dès lors, il est possible d'identifier des événements d'insertion spécifiques parmi la population considérée, en faisant appel à des sondes ou à des amorces spécifiques au gène. L'identification des mutants pour un gène particulier fournit des données concernant la fonction et les effets phénotypiques de ce gène, qui peut ensuite être utilisé dans la création de nouveaux produits en biotechnologie.

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