Method for selection of transformed cells

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 5/14 (2006.01)

Patent

CA 2653742

The invention provides methods for the selection of transgenic cells. The invention relates to the unexpected finding that cells expressing a gene conferring tolerance to auxin-like herbicides such as dicamba may be directly selected from non- transgenic cells using auxin-like herbicides as a selective agent. In this manner, plants exhibiting tolerance to auxin-like herbicides can be directly produced without the need for separate selectable markers.

La présente invention concerne des procédés de sélection de cellules transgéniques. L'invention porte sur la découverte inattendue que les cellules exprimant un gène conférant une tolérance aux herbicides de type auxine tels que le dicamba peuvent être directement sélectionnées parmi des cellules non transgéniques au moyen d'herbicides de type auxine en tant qu'agent sélectif. De cette manière, les plantes faisant preuve de tolérance vis-à-vis des herbicides de type auxine peuvent être directement produites sans le besoin de marqueurs sélectionnables séparés.

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