Method for selective methionine starvation of malignant...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/60 (2006.01) A61K 38/51 (2006.01) A61K 47/48 (2006.01) C12N 9/88 (2006.01) C12Q 1/527 (2006.01) C12Q 1/68 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2180262

An improved method for chemotherapy of mammalian malignant cells which have an absolute requirement for methionine but lack methylthioadenosine phosphorylase (MTAse). The method comprises detection of MTAse negative cells in a mammal administration of methionine .gamma.-lyase in sufficient amounts to reduce the volume of MTAse negative cells in the mammal, and co-administration of methylthioadenosine in amounts sufficient to ensure the continued availability of methionine to the mammal's non-malignant cells. Means for detection of MTAse negative cells are provided. Means for production and use of recombinant chemotherapeutic agents are also provided.

Est décrit un procédé perfectionné pour la chimiothérapie des cellules malignes mammaliennes qui présentent un besoin absolu de méthionine et qui manquent de méthylthioadénosine-phosphorylase (MTAse). Ce procédé consiste à détecter les cellules déficitaires en MTAse chez un mammifère, à lui administrer de la méthionine -.gamma.-lyase en quantités suffisantes pour réduire le volume des cellules déficitaires en MTAse chez ce mammifère, et à lui administrer conjointement de la méthylthioadénosine en quantités suffisantes pour assurer à ses cellules non malignes la présence constante de méthionine. Sont égalements décrits des moyens permettant la détection des cellules déficitaires en MTAse. Sont aussi décrits des moyens de production et d'utilisation d'agents chimiothérapeutiques recombinés.

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