Method for separating and characterising functions...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C12N 15/00 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2339904

The invention concerns a method for separating and characterising functions potentially present in a biological sample containing nucleic acids, characterised in that it comprises the following steps: (a) preparing nucleic acid fragments from said sample; (b) associating each of said fragments with a carrier molecule; (c) isolating each fragment or a part of each construct formed by a fragment associated with a carrier molecule; (d) treating in vitro each fragment or a part of each construct formed by a fragment associated with a carrier molecule isolated at step (c) to obtain transcripts; (e) testing a function of the transcripts obtained at step (d) or proteins for which they code after said transcripts have been translated.

La présente invention concerne un procédé de séparation et de caractérisation des fonctions potentiellement présentes dans un échantillon biologique contenant des acides nucléiques, caractérisé en ce qu'il comprend les étapes suivantes: (a) la préparation de fragments d'acide nucléique à partir dudit échantillon, (b) l'association de chacun desdits fragments avec une molécule vectrice, (c) l'isolement de chaque fragment ou d'une partie de chaque construction constituée par un fragment associé à une molécule vectrice, (d) le traitement in vitro de chaque fragment ou d'une partie de chaque construction constituée par un fragment associé à une molécule vectrice isolé à l'étape (c) pour obtenir des transcrits, (e) le test d'une fonction des transcrits obtenus à l'étape (d) ou des protéines pour lesquels ils codent après traduction desdits transcrits.

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