Method for separating and detecting proteins by means of...

G - Physics – 01 – N

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G01N 27/447 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2428372

The invention relates to a method for separating and detecting proteins or protein samples of cellular origin. Said proteins are contained in a separation buffer solution and the following steps are carried out: the protein samples are separated into individual fractions in a first separating step by means of free flow electrophoresis, isoelectric focusing (IEF) or isotachophoresis, and are linked with a marker; the protein fractions are separated into at least one capillary by means of capillary electrophoresis in a second separating step; and at least one marker linked with the protein fractions is detected in the at least one capillary.

L'invention concerne un procédé de séparation et de détection de protéines ou d'échantillons de protéines d'origine cellulaire, les protéines étant contenues dans une solution tampon de séparation, caractérisé en ce que, dans une première étape de la séparation, les échantillons de protéines sont séparés en fractions individuelles par électrophorèse à écoulement libre ou par focalisation isoélectrique (IEF) ou par isotachophorèse, et liés avec une substance de marquage, en ce que, dans une seconde étape, les fractions de protéines sont séparées, par électrophorèse capillaire, dans un ou plusieurs capillaires, et en ce que dans l'un ou plusieurs de ces capillaires, au moins une substance de marquage liée aux fractions de protéines est détectée.

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