G - Physics – 01 – N
Patent
G - Physics
01
N
150/19, 23/364
G01N 30/36 (2006.01) C07C 273/18 (2006.01) C07K 1/12 (2006.01) G01N 30/84 (2006.01) G01N 33/68 (2006.01) C07C 275/28 (2006.01)
Patent
CA 2037772
31,162-00 A METHOD FOR SEPARATING DIPHENYLUREA (DPU) AND PHENYLTHIOHYDANTOIN-TRYPTOPHAN (PTH-TRP) ALLOWING UNAMBIGUOUS IDENTIFICATION OF TRP IN AUTOMATIC SEQUENCE ANALYSIS ABSTRACT OF THE DISCLOSURE A reverse-phase HPLC method is disclosed which allows the separation of DPU and PTH-Trp and, therefore, the correct assignment of tryptophan residues in an amino acid sequence. The method is based on a modification of the conventional HPLC gradient commonly used to elute and separate all PTH amino acids of interest in automated and manual sequencing. In one embodiment, using automated instruments manufactured by Applied Biosystems, gradient modification is achieved by changing the manufacturer-supplied gradient program which controls the operation of the HPLC pumps in the instrument. Using the methods of the present invention, the correct and reliable assignment of tryptophan residues is possible and was reproducible over time, even with small sample sizes.
American Cyanamid Company
Mueller Thomas
Smart & Biggar
LandOfFree
Method for separating diphenylurea (dpu) and... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for separating diphenylurea (dpu) and..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for separating diphenylurea (dpu) and... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1999198