Method for separating highly active stem cells from human...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/0775 (2010.01)

Patent

CA 2732025

The present invention relates to a method for separating highly active stem cells from human stem cells, the highly active stem cells separated by the method, a cell therapeutic agent containing the stem cells, and a medium for separating the highly active stem cells from stem cells containing a specific cytokine. According to the present invention, the method is useful for separating the highly efficient stem cells from mesenchymal stem cells of various origins. Further, the method is very useful in developing a cell therapeutic agent of high efficiency because the method can be applicable to stem cells of various origins which are cultured under different conditions. Senescent stem cells increased by several passage times in vitro can be effectively sorted out, so the method can be used for reactivating the stem cells.

Cette invention concerne un procédé permettant de séparer des cellules souches humaines fortement actives de cellules souches humaines, les cellules souches fortement actives séparées par ce procédé, un agent de thérapie cellulaire contenant les cellules souches, et un milieu de séparation contenant un cytokine spécifique. D'après la présente invention, ce procédé convient pour la séparation de cellules souches hautement efficaces de cellules mésenchymateuses d'origines diverses. De plus, ce procédé convient particulièrement pour la mise au point d'un agent thérapeutique cellulaire d'une grande efficacité du fait qu'il peut être appliqué à des cellules souches de diverses origines cultivées dans des conditions différentes. Comme des cellules souches sénescentes accrues par plusieurs temps de passage in vitro peuvent être effectivement triées, le procédé peut servir à réactiver les cellules souches.

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