C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
182/2, 195/1.12
C12N 15/10 (2006.01) C07H 1/08 (2006.01) C07H 21/00 (2006.01) G01N 30/60 (2006.01) G01N 30/88 (2006.01)
Patent
CA 1339772
METHOD FOR SEPARATING LONG-CHAIN NUCLEIC ACIDS Long-chain nucleic acids are separated from other substances from solutions containing nucleic acids and other materials, and more particularly nucleic acid/protein mixtures from biotechnical preparations from bacteria, viruses, animal and vegetable tissues and cells as well as body liquids, more particularly cell ingredients and/or degradation products thereof as well as components of body liquids which components are not long-chain nucleic acids, by that the long-chain nucleic acids in the nucleic acid-containing solutions, or after disintegration under mild conditions of the tissue cells and/or cells from body liquids are fixed on a porous matrix, whereas the substances to be separated therefrom are washed out from the matrix, and the fixed nucleic acids, if desired, are subsequently removed from the matrix. A device for carrying out the method preferably consists of a cartridge (1) containing the porous matrix (11) and having at least one inlet opening (3) and at least one outlet opening (4).
552250
Colpan Metin
Henco Karsten
Stichel Arndt
Diagen Institut Fur Molekularbiolgische Diagnostik Gmbh
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
Qiagen Gmbh
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