C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
6/64, 195/39.1
C12N 9/36 (2006.01) C08L 5/00 (2006.01) C08L 5/12 (2006.01)
Patent
CA 1221046
ABSTRACT OF THE DISCLOSURE The present invention relates to a method for separating lysozyme from egg-white which has a lysozyme fraction and an albumin fraction, without destroying the commercial utility of the lysozyme fraction or the albumin fraction. The method comprises the step of preferentially, reversibly binding the lysozyme fraction of the egg-white to an affinity resin at about the natural pH of the egg-white by contacting the egg-white with the resin, separating the albumin fraction from the resin to leave the lysozyme fraction bound to the resin in a lysozyme- resin complex, and eluting the bound lysozyme from the resin to obtain a lysozyme solution and reusable resin. The eluted lysozyme and the separated albumin fraction are further treated in separating processing lines to produce a commercial albumin fraction egg-white product and a commercial lysozyme product. The invention also deals with the preparation and compositions of suitable affinity resins for the reversible, preferential binding of lysozyme in egg white.
455498
Brookside Farms Ltd.
Frayne & Company Robert
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