Method for separating nucleic acids into populations

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) C12N 15/09 (2006.01) C12N 15/10 (2006.01) G01N 33/53 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2353103

A method for at least partially separating nucleic acid molecules in a sample into populations wherein a population is tagged or capable of being tagged with a moiety capable of being immobilised on a matrix, said method comprising contacting the nucleic acid containing sample with a matrix whereby the tagged molecules are captured by the matrix and thereby separated from untagged molecules. The method may be used in a variety of applications, including separating restriction enzyme digestion products, separating linear from circular nucleic acid molecules, in vitro packaging of bacteriophage particles, diagnostic PCR.

La présente invention concerne un procédé permettant de séparer au moins en partie des molécules d'acides nucléiques contenues dans un échantillon en populations. L'une de ces populations est marquée ou peut être marquée avec une fraction pouvant être immobilisée dans une matrice. Ce procédé consiste à mettre en contact l'échantillon contenant l'acide nucléique avec une matrice, les molécules marquées étant capturées par la matrice et séparées des molécules non marquées. En outre, on peut utiliser ce procédé dans une variété d'applications, notamment pour séparer les produits de digestion d'enzymes de restriction, pour séparer les molécules d'acides nucléiques linéaires et circulaires, pour encapsuler in vitro des particules bactériophages, et dans le diagnostic PCR.

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