Method for separating off viruses from a protein solution by...

Details Method for separating off viruses from a protein solution by... Method for separating off viruses from a protein solution by...

C - Chemistry, Metallurgy

07

K

C07K 1/34 (2006.01) A61L 2/00 (2006.01) C07K 14/75 (2006.01)

Patent

CA 2421681

A method for separating off viruses from a protein solution by means of nanofiltration is described, in which a chaotropic substance from the group consisting of arginine, guanidine, citrulline, urea or their derivatives, or a compound from the polyethoxysorbitan ester group, is added to the protein solution, prior to the nanofiltration, in order to decrease or prevent aggregation of the protein molecules, and the solution is then passed through a filter having a pore size of between 15 and 25 nm.

Une méthode permettant de séparer les virus d'une solution de protéines au moyen d'une nanofiltration est décrite. Dans cette méthode, une substance chaotropique choisie parmi l'arginine, la guanidine, la citrulline, l'urée ou leurs dérivés, ou un composé du groupe des esters de polyéthoxysorbitane, est ajoutée à la solution protéinique, avant la nanofiltration, afin de diminuer ou de prévenir l'agrégation des molécules de protéine, et la solution est passée à travers un filtre dont la taille des pores se situe entre 15 et 25 nm.

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