Method for separation of dextrans

C - Chemistry – Metallurgy – 08 – B

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C08B 37/02 (2006.01)

Patent

CA 1088056

ABSTRACT The invention described and claimed in the specification is a new method for separation of a fluid mixture of dextrans of differing molecular weights into two fractions, which comprises (a) passing said mixture into chromatographic apparatus which comprises a plurality of compartments which contain an appropriate packing material, valve-containing conduits interconnecting the compartments to provide a closed loop column, at least one additional valve controlled fluid inlet and at least one additional valve controlled fluit outlet for each compartment, and means for sequential operation of the valves, and (b) operating the valves sequentially such that each compartment undergoes, and at any one time there is at least one compartment undergoing, the following operations: (i) being fed with the fluid mixture to be separated, (ii) being fed with a carrier fluid, (iii) having carrier fluid and any entrained faster moving components of the fluid mixture removed therefrom, and (iv) being isolated and treated to remove any slower moving components of the fluid mixture; the compartment being fed with fluid mixture to be separated being a compartment through which the carrier fluid passes.

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