Method for separation of hemoglobin a.sub.1.sub.c

Details Method for separation of hemoglobin a.sub.1.sub.c Method for separation of hemoglobin a.sub.1.sub.c

G - Physics

01

N

150/11, 167/114

G01N 33/72 (2006.01) A61K 35/18 (2006.01) G01N 33/66 (2006.01)

Patent

CA 1189774

ABSTRACT OF THE DISCLOSURE A novel ion exchange method is provided for the separation of hemoglobin AlC from other hemoglobin com- ponents in a sample of human blood. According to the dis- closed method, the sample is lysed then used to impregnate a weak cation exchanger. Two buffer solutions are then passed through the column in succession, the first having an alkali metal ion concentration of from about 0.02M to about 0.05M and the second having an alkali metal ion concentra- tion of from about 0.06M to about 0.11M. The second eluate contains substantially all of the hemoglobin AlC and sub- stantially none of the other hemoglobin components in the original sample. Analysis of the second eluate thus pro- vides a reliable indication of the long-term glucose level in the blood of a patient, and hence the patient's ability to regulate the quantity of glucose ingested.

424599

Affiliated with

Also associated with

Rating

Method for separation of hemoglobin a.sub.1.sub.c does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for separation of hemoglobin a.sub.1.sub.c, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for separation of hemoglobin a.sub.1.sub.c will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1333927