Method for sequencing of nucleic acid polymers

Details Method for sequencing of nucleic acid polymers Method for sequencing of nucleic acid polymers

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2266755

Sequencing of a selected region of a target nucleic acid polymer in a natural abundance DNA sample can be performed in a single vessel by combining the sample with a sequencing mixture containing a primer pair, a thermally stable polymerase such as Thermo Sequenase TM which incorporates dideoxynucleotides into an extending nucleic acid polymer at a rate which is no less than about 0.4 times the rate of incorporation of deoxynucleotides, nucleotide feedstocks, and a chain terminating nucleotide. The reaction mixture also includes an unconventional nucleotide and an appropriate enzyme for degradation of nucleic acid polymers containing the unconventional nucleotide. The mixture is processed through multiple thermal cycles for annealing, extension and denaturation to produce a product mixture which is analyzed by electrophoresis.

Affiliated with

Also associated with

Rating

Method for sequencing of nucleic acid polymers does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for sequencing of nucleic acid polymers, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for sequencing of nucleic acid polymers will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1603327