Method for serial analysis of gene expression

C - Chemistry – Metallurgy – 12 – P

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C12P 19/34 (2006.01) C07H 21/00 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2185379

Serial analysis of gene expression, SAGE, a method for the rapid quantitative and qualitative analysis of transcripts is provided. Short defined sequence tags correspond- ing to expressed genes are isolated and analyzed. Sequencing of over 1,000 defined tags in a short period of time (e.g, hours) reveals a gene expression pattern characteris- tic of the function of a cell or tissue. Moreover, SAGE is useful as a gene discovery tool for the identification and isolation of novel sequence tags corresponding to novel transcripts and genes.

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