Method for solubilizing microbial coatings and protein...

Details Method for solubilizing microbial coatings and protein... Method for solubilizing microbial coatings and protein...

G - Physics

01

N

167/135

G01N 33/531 (2006.01) G01N 33/569 (2006.01)

Patent

CA 1242391

ABSTRACT A method of specimen treatment preparatory to conducting an immunoassay is disclosed whereby a microbial protein is solubilized by a detergent at elevated temperatures and in the presence of an alkali or alkaline earth metal ion. At elevated temperatures, the detergent is soluble. However, at lower tempera- tures, the presence of the metal ion renders the deter- gent insoluble so that it is prevented from interacting in the immunoassay procedure. A specific application is in the solubilization of the principal outer membrane protein of Chlamydia trachomatis.

484930

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