Method for solution based diagnosis

Details Method for solution based diagnosis Method for solution based diagnosis

G - Physics

01

N

G01N 33/574 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2487048

This invention provides methods for improved diagnosis of medically relevant conditions by solution based biochemical testing procedures performed in solutions of test samples. The invention provides a method to substitute the cell based morphological information contained within the cytological and/or histological data of the test sample by molecular information obtainable from the solution, wherein the original test sample is dissolved and thus enables for accurate and reproducible assessment of medically relevant diagnosis from dissolved test samples. The method according to the invention comprises the steps of determining the levels of one or more disease markers associated with the condition to be diagnosed, determining the level of one or more normalization markers suitable to substitute the information related to morphological aspects of the sample, comparing and/or combining the data of the disease and normalization markers, and assessing diagnosis of a medically relevant condition.

L'invention concerne des procédés destinés à améliorer le diagnostic de troubles pertinents sur le plan médical à l'aide de procédures d'essai biochimiques à base de solution exécutée en solutions d'échantillons d'essai. L'invention concerne également un procédé destiné à substituer les informations morphologiques basées sur la cellule contenue dans les données cytologiques et/ou histologiques de l'échantillon d'essai au moyen d'informations moléculaires pouvant être obtenues à partir de la solution, l'échantillon d'essai original étant dissolu ce qui permet un examen précis et reproductible de diagnostic pertinent sur le plan médical à partir des échantillons d'essai dissolus. Selon l'invention, le procédé comprend les étapes consistant à déterminer les niveaux d'un ou plusieurs marqueurs de maladies associés au trouble à diagnostiquer, à déterminer le niveau d'un ou plusieurs marqueurs de normalisation capable(s) de substituer les informations relatives aux aspects morphologiques de l'échantillon, à comparer et/ou à combiner les données de la maladie et les marqueurs de normalisation, et à examiner le diagnostic d'un trouble pertinent sur le plan médical.

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