Method for stabilization of enzymes during exposure to...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 9/96 (2006.01)

Patent

CA 2525758

The present invention provides a composition comprising an enzyme, e.g. oxidase enzyme, a source of zinc and/or ammonium ions and a source of lactate ions. The compositions are typically sterilised by exposing the compositions to sterilising radiation, e.g. gamma radiation. The incorporation of a source of zinc and/or ammonium ions and a source of lactate ions, e.g. zinc L- lactate, in the enzyme-containing composition results in an improvement in enzyme activity post-sterilisation. The presence of a source of zinc and/or ammonium ions and a source of lactate ions in the composition therefore has a protective effect on the enzyme during exposure to sterilising radiation so that good recovery of enzyme activity can be obtained.

L'invention concerne une composition comprenant une enzyme, par exemple une enzyme oxydase, une source d'ions zinc et/ou ammonium et une source d'ions lactates. Les compositions sont d'ordinaires stérilisées par exposition à un rayonnement stérilisant, par exemple un rayonnement gamma. L'incorporation d'une source d'ions zinc et/ou d'ammonium et d'une source d'ions lactates, par exemple L-lactate de zinc, dans la composition contenant l'enzyme produit une amélioration de l'activité enzymatique post-stérilisation. La présence d'une source d'ions zinc et/ou ammonium et d'une source d'ions lactates dans la composition produit par conséquent un effet protecteur sur l'enzyme pendant son exposition au rayonnement stérilisant, ce qui permet d'obtenir une bonne récupération de l'activité enzymatique.

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