C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/09 (2010.01) A01N 1/02 (2006.01) C12N 1/04 (2006.01) C12Q 1/02 (2006.01) G01N 33/50 (2006.01) A61K 9/127 (2006.01)
Patent
CA 2523417
The present invention relates to a method for storing cells, wherein the cells are stored in a composition comprising a base nutritive medium and liposomes at temperatures in the range of -196~C to 37~C, characterized in that the liposomes comprise one or more sterols and that the cells are tumor cells. More specifically, the invention relates to simple and effective methods for storing tumor cells, wherein the RNA and/or DNA in the cells is essentially not degraded, such that the RNA and/or DNA can be analyzed after storage.
La présente invention concerne une méthode de stockage de cellules consistant à stocker des cellules dans une composition comprenant un milieu nutritif de base et des liposomes à des températures comprises entre 196 ·C et 37 ·C. L'invention se caractérise en ce que les liposomes comprennent un ou plusieurs stérols et en ce que les cellules sont des cellules tumorales. Plus particulièrement, l'invention concerne des méthodes simples et efficaces destinées à stocker des cellules sensiblement sans dégradation de leur ARN et/ou de leur ADN, l'ARN et/ou l'ADN pouvant ainsi être analysés après stockage.
Bach Ferdinand
Fischer Joseph
Gowling Lafleur Henderson Llp
Oncoscience Ag
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