C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 1/20 (2006.01) A23C 9/123 (2006.01) A23C 19/032 (2006.01) C12M 1/00 (2006.01) C12N 1/16 (2006.01)
Patent
CA 2403398
The present invention relates to the field of producing starter cultures. In particular, a method for customers in need of a starter culture with a consistent quality, is provided. Specifically, the method involves the use of subsets of a stock inoculum material, which comprises a concentrate of starter culture organism cells to be propagated for direct inoculation of a cultivation medium, to obtain a starter culture whereby the conventional stepwise preparation of inoculum material for the production of a starter culture can be avoided. This novel method can be used for the manufacturing of starter cultures for the food, feed or pharmaceutical industry. Furthermore, the method is useful in the cultivation of cells expressing desired products, such as primary and secondary metabolites, including e.g. enzymes and flavours.
L'invention concerne la production de cultures starters, et notamment un procédé pour les clients ayant besoin de cultures starters de qualité constante. Selon ce procédé, on utilise des sous-ensembles d'un inoculum de départ comprenant un concentré de cellules organiques de culture starter, qui se multiplient pour être directement inoculées dans un milieu de culture, en vue d'obtenir une culture starter. On peut éviter ainsi la préparation classique d'inoculum par étapes pour la production d'une culture starter. Ce nouveau procédé peut être appliqué pour produire des cultures starters destinées aux industries de l'alimentation humaine, de l'alimentation animale et pharmaceutique. En outre, ledit procédé s'avère utile pour cultiver des cellules exprimant les produits désirés comme des métabolites primaires et secondaires, comprenant, par exemple, des enzymes et des saveurs.
Kringel Maibritt
Kringelum Borge
Nielsen Knud Striib
Blake Cassels & Graydon Llp
Chr. Hansen A/s
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