C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/00 (2006.01) C12P 19/34 (2006.01) C12Q 1/00 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2184270
Methods and kits for the use of a non-ionic detergent to suppress enzyme inhibition in a reaction solution due to the presence of inhibiting ionic detergent. Prior to reaction, the reaction mixture is given an effective amount of a non-ionic detergent, and agitated. The enzyme is then added, and the enzymatic reaction is then allowed to proceed. Also disclosed are preferred embodiments of the present invention, and kits for nucleic acid amplification of a biological sample in the presence of an ionic detergent.
Des procédés et des trousses permettent d'utiliser un détergent non ionique pour réprimer une inhibition enzymatique intervenant dans une solution de réaction à cause d'un détergent ionique inhibiteur. Avant toute réaction, on ajoute au mélange de réaction une quantité efficace d'un détergent non ionique et on procède à une agitation. On ajoute ensuite l'enzyme, ce qui provoque la réaction enzymatique. On décrit aussi des variantes préférées de l'invention, ainsi que des trousses d'amplification d'acides nucléiques contenus dans un échantillon biologique en présence d'un détergent ionique.
Kacian Daniel Louis
Mcallister Diane Lisa
Gen-Probe Incorporated
Smart & Biggar
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