Method for suppressing nonspecific hybridization in primer...

C - Chemistry – Metallurgy – 12 – P

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C12P 19/34 (2006.01) C07H 21/00 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2206451

An object of the present invention is to suppress nonspecific extension reaction of the primer in the primer extension method. The primer extension reaction to form a nucleic acid strand complementary to a nucleic acid template strand with the use of a primer according to the present invention is characterized in that the reaction between the primer and the template strand is carried out in the presence of a nucleic acid or a derivative thereof which is complementary to said primer and has an affinity for said primer is equivalent to or less than that of said primer for the nucleic acid template strand.

Procédé d'inhibition de l'hybridation non spécifique d'une amorce au cours de la réaction d'extension d'amorce destinée à former un brin d'acide nucléique complémentaire au brin complémentaire d'un acide nucléique à l'aide d'une amorce, caractérisé en ce qu'une réaction de l'amorce avec le brin complémentaire est réalisée en présence d'un acide nucléique ou d'un dérivé de ce dernier qui est complémentaire à l'amorce et qui présente une affinité, pour l'amorce, égale ou inférieure à l'affinité de l'amorce pour le brin complémentaire de l'acide nucléique.

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