C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 21/02 (2006.01) C12P 21/00 (2006.01)
Patent
CA 2365237
The method of polypeptide synthesis in eukaryotic or porkaryotic cell-free systems based on a modified version of synthesis in the continuous flow or continuous exchange modes when, in addition to input into the reaction mixture of components maintaining the synthesis and removal from the reaction mixture of low molecular weight components inhibiting the synthesis, the concentration of at least one of the components selected from the group consisting of Mg2+, K+, NTP, polyamines or their combinations determining the productivity of the synthesis is continuously changed within the given range of concentrations, while the concentrations of the other components are maintained constant.
Technique permettant de synthétiser des polypeptides dans des systèmes acellulaires exempts de cellules eucaryotes ou procaryotes, basée sur une version modifiée de la synthèse en flux continu ou en échange continu. Selon la technique, on ajoute au mélange de réaction des composants qui maintiennent la synthèse et on retire dudit mélange les composants de faible poids moléculaire qui inhibent la synthèse; et on fait varier en continu, à l'intérieur de la plage de concentration prédéterminée, la concentration d'au moins un des composants, choisis dans le groupe comprenant Mg<2+>, K<+>, les NTP, les polyamines ou leurs combinaisons, qui conditionnent la productivité de la synthèse, tout en maintenant constante la concentration des autres composants.
Biryukov Sergey Vladimirovich
Shirokov Vladimir Anatolievich
Simonenko Peter Nikolaevich
Spirin Alexander Sergeyevich
Borden Ladner Gervais Llp
Institute Of Protein Research
Roche Diagnostics Gmbh
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