C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/76 (2006.01) C12N 9/64 (2006.01) C12N 15/09 (2006.01) C12N 15/57 (2006.01) C12P 21/00 (2006.01) C12P 21/02 (2006.01)
Patent
CA 2423294
The method for producing peptides, peptide mimetics and proteins is characterized in that an enzyme is used as a biocatalyst together with a peptide mimetic. The native specificity of the enzyme is modified by chemical or genetic manipulation, the substrate mimetic is a carboxyl component with an ester leaving group whose specificity determinant is adapted to the recognition site of the enzyme. The invention also concerns the trypsin variant D189K,K60E and its use as a C-N ligase in segment condensations.
L'invention concerne un procédé de production de peptides, peptides mimétiques et protéines caractérisé en ce que l'on utilise comme biocatalyseur une enzyme avec un substrat mimétique. La spécificité d'origine de l'enzyme est modifiée par manipulation chimique ou génétique. Le substrat mimétique est un composant carboxyle comportant un groupe partant ester, dont le déterminant de spécificité est adapté à la région de reconnaissance de l'enzyme. L'invention concerne également les variantes de la trypsine D189K,K60E et leur utilisation comme ligase CN dans des condensations de segments.
Bordusa Frank
Jakubke Hans-Dieter
Borden Ladner Gervais Llp
F. Hoffmann-La Roche Ag
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