Method for synthesizing single-stranded stem-loop dnas, the...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/11 (2006.01) C12N 15/56 (2006.01) C12N 15/70 (2006.01) C12P 19/34 (2006.01) C40B 40/06 (2006.01)

Patent

CA 2073630

A method of synthesis of new and useful single-stranded DNAs which have a stem-loop configuration (ss-s1DNA). The method is an in vivo or an in vitro synthesis. Replicating vehicles which produce these ss-s1DNAs. The ss-s1DNAs are described. Uses for these s1DNAs are disclosed. They can be used for introducing random mutations, they lend themselves for replication by a variant of the PCR method. Other uses are disclosed.

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