Method for synthesizing stable single-stranded cdna in...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/79 (2006.01) C07H 21/00 (2006.01) C12N 9/12 (2006.01) C12N 15/10 (2006.01) C12N 15/11 (2006.01) C12N 15/54 (2006.01) C12N 15/81 (2006.01)

Patent

CA 2075515

A method for producing in vivo stable single-stranded DNAs in eucaryotic cells. The DNAs are multicopy single-stranded DNA (msDNA) structures constituted by a RNA and a DNA portion. The group of genes (retroris) producing said coupled RNA and DNA portions of the msDNAs and the gene encoding reverse transcriptase (RT). The transformed eucaryotes harboring these retrons. The new msDNAs which are encoded by the new retrons. Utilities are disclosed.

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