C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2390309
An oligonucleotide having a novel structure and a method for synthesizing a nucleic acid by using the same as a primer. In the 5'-side of the primer, this oligonucleotide is provided with a base sequence which is substantially identical with the region to be synthesized by using this primer as a starting point of the synthesis. Thus, a nucleic acid can be synthesized on the basis of an isothermal reaction with the use of a simple reagent constitution. Also, provision is made of a method for synthesizing a nucleic acid having a high specificity based on the above method for synthesizing a nucleic acid.
L'invention porte sur un oligonucléotide présentant une structure nouvelle et sur un procédé de synthèse d'un acide nucléique l'utilisant comme amorce. L'oligonucléotide du côté 5' de l'amorce comporte une séquence de base sensiblement identique à la région à synthétiser lorsqu'on utilise ladite amorce comme point de départ de la synthèse. On peut ainsi synthétiser un acide nucléique sur la base d'une réaction isothermique en recourant à une simple constitution de réactif. L'invention porte également sur un procédé de synthèse d'un acide nucléique à forte spécificité sur la base du procédé ci-dessus de synthèse d'un acide nucléique.
Hase Tetsu
Notomi Tsugunori
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Eiken Kagaku Kabushiki Kaisha
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