Method for testing a cell sample

Details Method for testing a cell sample Method for testing a cell sample

G - Physics

01

N

G01N 33/487 (2006.01) G01N 13/00 (2006.01) G01N 13/04 (2006.01) G01N 15/00 (2006.01) G01N 15/08 (2006.01) G01N 15/10 (2006.01) G01N 33/49 (2006.01) G01N 33/483 (2006.01)

Patent

CA 2240471

In this invention, a measurement of cell permeability is determined by obtaining a measure of the volume of fluid which crosses a sample cell membrane in response to an altered environment. A lytic agent may be used to drive fluid across the cell membranes and thereby cause a change in cell volume. An alteration in osmolality of a sample suspension is preferred, in which the sample suspension is subjected to a continuous osmotic gradient.

L'invention concerne la mesure de la perméabilité cellulaire, déterminée par obtention d'une mesure du volume de fluide qui traverse la membrane des cellules d'un échantillon, en réponse à un environement modifié. Un agent lytique peut être utilisé pour faire passer le fluide à travers les membranes cellulaires et provoquer ainsi un changement du volume des cellules. On préfère une modification de l'osmolalité d'une suspension de l'échantillon, dans laquelle la suspension de l'échantillon est soumise à un gradient osmotique continu.

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