G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/53 (2006.01) C12Q 1/37 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2437880
The invention relates to a method for testing samples containing prion protein for the possible presence of the PrPSc form, according to which: (Step a) the sample is mixed with protease in order to digest protease-sensitive proteins or protein regions; (Step b) after digestion, it is tested whether the sample contains the region PrP 27-30 of the prion protein, which is resistant in the PrPSc form of the prion protein protease, and the presence of PrPSc in the sample is established based on the positive detection of PrP 27-30. The inventive method is characterized in that, during Step b, the sample is additionally tested in order to determine whether a complete digestion of the protease-sensitive region of the prion protein has occurred.
L'invention concerne un procédé d'étude d'échantillons contenant la protéine du prion quant à l'éventuelle présence de la forme PrP?Sc¿, consistant (a) à mélanger des protéases à l'échantillon pour la digestion de protéines ou de zones protéiques sensibles aux protéases, (b) à vérifier après digestion si l'échantillon contient la zone PrP 27-30 de la protéine du prion résistant aux protéases dans la forme PrP?Sc¿ de la protéine du prion, et à identifier la présence de PrP?Sc¿ dans l'échantillon en cas de détection de PrP 27-30. Ledit procédé est caractérisé en ce que l'étape (b) consiste par ailleurs à vérifier dans l'échantillon s'il y a eu digestion complète de la zone de la protéine du prion sensible aux protéases.
Biffiger Karin
Moser Markus
Oesch Bruno
Perley-Robertson Hill & Mcdougall Llp
Prionics Ag
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