Method for the cryopreservation of cells, artificial cell...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/077 (2010.01)

Patent

CA 2751985

The present invention belongs to the field of cryopreservation of cells and tissue cultures, more specifically it refers to a method for the cryopreservation and long term storage of cells, cell constructs or three-dimensional complex tissues assemblies. The methods are based on the use of a collagen cell carrier having a specific composition as well as to a very specific thickness and appropriate mechanical properties which are maintained after thawing. The use of such collagen cell carrier (CCC) provides a very suitable support for the cryopreservation resulting in very high survival rates after thawing and providing cells and tissue assemblies already adhered to a mechanically stable and biocompatible support. The invention also refers to the frozen collagen carrier-cells assembly and to the frozen artificial cell constructs or three- dimensional complex tissue assemblies obtainable by the method of the invention and to the use thereof after thawing.

La présente invention concerne le domaine de la cryoconservation de cultures cellulaires et tissulaires, plus spécifiquement, l'invention concerne un procédé de cryoconservation et de stockage à long terme de cellules, de constructions cellulaires ou d'ensembles tissulaires tridimensionnels complexes. Les procédés sont fondés sur l'utilisation d'un support cellulaire de collagène présentant une composition spécifique de même qu'une épaisseur bien spécifique et des propriétés mécaniques appropriées qui persistent après décongélation. L'utilisation d'un tel support cellulaire de collagène (SCC) permet d'obtenir un support très approprié à la cryoconservation conduisant à un taux de survie très élevé après décongélation et garantissant des ensembles cellulaires et tissulaires déjà adhérés à un support mécaniquement stable et biocompatible. L'invention concerne également l'ensemble support de collagène-cellules congelé et les constructions cellulaires artificielles congelées ou les ensembles tissulaires tridimensionnels complexes congelés pouvant être obtenus grâce au procédé de l'invention, ainsi que leur utilisation après décongélation.

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