C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 1/10 (2006.01) A23K 1/16 (2006.01) C12N 1/12 (2006.01) C12N 1/14 (2006.01) C12P 7/64 (2006.01)
Patent
CA 2545410
The invention relates to an optimized method for cultivating microorganisms of the genus thraustochytriales, according to which the microorganisms are cultivated in a low salt medium without adding sodium salts and chloride salts, the total salt content being less than 3.5g/L (corresponding to less than 10 percent of sea water content), whereupon the PUFAs are isolated from the microorganisms and/or the medium. The invention especially relates to novel optimized media having a substantially reduced total salt content, above all a particularly reduced NaCl content. The production of PUFAs can be substantially improved and significantly simplified by using a novel combination of different salts as a media composition in which the overall weight ratios of Na+ and Cl- ions do not exceed 1.75 g/L. Furthermore, said medium preferably contains no added sodium salt and chloride salt at all, which helps prevent environmental damages caused by wastewaters containing salt.
L'invention concerne un procédé optimisé pour cultiver des micro-organismes de l'ordre des Thraustochytriales, selon lequel les micro-organismes sont cultivés dans un milieu faiblement salin sans addition de sel de sodium ni de sel de chlorure, pour une teneur totale en sel inférieure à 3,5g/L (correspondant à moins de 10 % de la teneur en sel de l'eau de mer), les acides gras polyinsaturés (PUFA) étant ensuite isolés des micro-organismes et/ou du milieu. L'invention concerne en particulier de nouveaux milieux optimisés à teneur totale en sel et en NaCl sensiblement réduite. Selon l'invention, une nouvelle combinaison de différents sels en tant que composition d'un milieu contenant, dans la somme des pourcentages en poids, pas plus de 1,75 g/L d'ions Na?+¿ et d'ions Cl?-¿, permet de perfectionner et de simplifier notablement la production des PUFA. En outre, le milieu décrit est de préférence totalement exempt d'additions de sel de sodium et de sel de chlorure, ce qui contribue à éviter la pollution environnementale par des eaux usées salées.
Luy Markus
Rusing Matthias
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Nutrinova Nutrition Specialties & Food Ingredients Gmbh
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