C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 7/00 (2006.01) A61K 39/12 (2006.01) A61K 39/275 (2006.01) A61P 31/12 (2006.01) C12N 5/071 (2010.01)
Patent
CA 2494379
The present invention relates to a method for the cultivation of primary cells. The primary cells are cultivated in a serum free medium comprising a factor selected from the group consisting of growth factors and attachment factors. The method for the cultivation of primary cells may be one step in a method for the amplification of viruses, such as poxviruses. According to this latter method the primary cells are cultivated in a serum free medium comprising a factor selected from the group consisting of growth factors and attachment factors. The cells are then infected with the virus and the infected cells are cultivated in serum free medium until progeny virus is produced.
Cette invention a trait à une technique de culture de cellules primaires. On cultive ces cellules primaires dans un milieu exempt de sérum, contenant un facteur choisi entre des facteurs de croissance et des facteurs de fixation. Cette culture de cellules primaires peut constituer une étape de l'amplification de virus, notamment de poxyvirus. Dans le cadre de cette technique, on cultive les cellules primaires dans un milieu exempt de sérum, contenant un facteur choisi entre des facteurs de croissance et des facteurs de fixation. On infecte ensuite les cellules au moyen d'un virus et on les cultive dans un milieu exempt de sérum jusqu'à l'obtention de virus de descendance.
Felder Eva
Heller Karl
Raethe Ingmar
Bavarian Nordic A/s
Smart & Biggar
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