G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/543 (2006.01)
Patent
CA 2526744
The invention relates to a method for the detection and multiplex quantification of analytes in a sample, using functionalised microspheres, whereby said microspheres are magnetised after the sample has been brought into contact therewith. The inventive method is particularly suitable for the detection and multiplex quantification of several analytes by means of flow cytometry. The invention also relates to a kit which is used for the detection and/or quantification of several analytes in order to carry out the inventive method, comprising a suspension of functionalised non-magnetic microspheres, a ferrofluid solution and a solution with at least one conjugate.
L~invention a pour objet un procédé de détection et/ou de quantification multiplex d~analytes dans un échantillon à l~aide de microsphères fonctionnalisées, ces microsphères étant magnétisées après l~étape de mise en présence de l~échantillon avec ces microsphères. Le procédé de l~invention est particulièrement adapté à la détection et à la quantification multiplex de plusieurs analytes par cytométrie de flux. L~invention à également pour objet un kit pour la détection et/ou la quantification de plusieurs analytes pour la mise en oeuvre du procédé selon l~invention qui comprend une suspension de microsphères non magnétiques fonctionnylisées, une solution de ferrrofluides et une solution d~au moins un conjugué.
Canton Michel
Haquette Guillaume
Moulard Maxime
Poncelet Philippe
Biocytex
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
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