C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12P 19/34 (2006.01) C12N 15/113 (2010.01)
Patent
CA 2726378
In a first aspect, the present invention relates to a method for the detection of a RNA molecule, said method comprising the steps of providing a sample containing the RNA molecule; hybridizing to said RNA molecule a first polynucleotide; extending the first polynucleotide to generate a first strand cDNA; hybridizing a second polynucleotide to the first strand cDNA; extending the first strand cDNA to generate an extension reaction product; amplifying the extension reaction product by means of polymerase chain reaction; and detecting the amplification product by means of real-time fluorescence readout. In a further aspect, the present invention relates to a kit comprising a first and a second polynucleotide as defined in the present invention, a set of dNTPs, a reverse transcriptase enzyme, and a detection moiety. In another aspect, the present invention relates to the use of said kit for the detection of an RNA molecule.
Albers Andreas
Ankenbauer Waltraud
Bergmann Frank
Froehner Stefanie
Heindl Dieter
Borden Ladner Gervais Llp
F. Hoffmann-La Roche Ag
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