Method for the detection of abnormally glycosylated proteins

G - Physics – 01 – N

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G01N 33/74 (2006.01) G01N 33/76 (2006.01)

Patent

CA 2534815

A method of screening a sample for the presence of one or more abnormally glycosylated and/or expressed proteins comprises the steps of i) exposing said sample to two or more différent lectins and/or antibodies ii) determining the extent of binding of said sample to at least two of said lectins and/or antibodies and iii) comparing the determined extent of binding to said at least two lectins and/or antibodies with that of a control sample.

La présente invention concerne un procédé pour cribler un échantillon pour détecter la présence d'une ou plusieurs protéines à glycosylation et/ou expression anormale, le procédé comprenant les étapes suivantes: i) exposition dudit échantillon à au moins deux lectines et/ou anticorps différent(e)s; ii) détermination de l'importance de la liaison dudit échantillon aux deux ou plus desdit(e)s lectines et/ou anticorps; iii) et comparaison de l'importance déterminée de la liaison aux deux ou plus desdit(e)s lectines et/ou anticorps avec celle d'un échantillon témoin.

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