Method for the detection of amyloid-.beta. oligomers in body...

G - Physics – 01 – N

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G01N 33/68 (2006.01)

Patent

CA 2693212

The present invention relates to a method for the detection of marker of the Alzheimer's disease, namely the amy- loid-.beta. oligomers in human CSF, using a combination of steps including demasking the epitopes responsible for antibody binding on the A.beta. peptide oligomers as well as detecting fluorescently marked antibodies binding to said epitopes, preferably by using the FRET technology.

La présente invention concerne une méthode de détection de marqueur de la maladie d'Alzheimer, à savoir les oligomères bêta-amyloïdes présents dans le liquide céphalorachidien (LCR) humain, par une combinaison d'étapes consistant à démasquer les épitopes responsables de la liaison des anticorps aux oligomères de peptide Aß, ainsi qu'à détecter des anticorps marqués par fluorescence liés à ces épitopes, de préférence par l'utilisation de la technique FRET.

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