Method for the detection of an analyte by immunochromatography

G - Physics – 01 – N

Patent

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G01N 33/558 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2236134

Disclosed is an improved method for the detection of an analyte in a fluid test sample using a strip of a negatively charged matrix material having a zone containing mobile, labeled binding partner for the analyte and a separate zone for capturing the labeled binding partner as it is carried through this zone by the fluid test sample. The improvement involves combining the fluid test sample with a polyalkoxylated amine surfactant to control non-specific binding of the labeled binding partner to the negatively charged matrix material.

Divulgation concernant une méthode pour la détection d'un produit dans un échantillon liquide à analyser, utilisant une bande avec matrice de matière chargée négativement, constituée d'une zone contenant un composé de liaison mobile, marqué, pour le produit à analyser, et une zone séparée pour capter le composé de liaison marqué, à mesure qu'il est transporté à travers cette zone par l'échantillon liquide à analyser. L'amélioration consiste à combiner l'échantillon liquide à analyser avec un tensioactif à base d'amine polyalcoxylée empêchant la liaison non spécifique du composé de liaison marqué avec la matrice de matière chargée négativement.

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