Method for the detection of creatinine

G - Physics – 01 – N

Patent

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G01N 33/70 (2006.01) G01N 33/52 (2006.01)

Patent

CA 2198957

Disclosed is a method for the determination of creatinine in urine which involves contacting a urine test sample with a reagent system containing Cu+2, a hydroperoxide and an oxidizible dye which provides a detectable response when oxidized in the presence of a pseudoperoxide. The reagent system also includes a buffer to maintain the urine's pH within the range of 6.6 to 8.0 and a chelating agent for the cupric ions to prevent oxidation of the dye in the absence of creatinine.

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