Method for the detection of cytochrome b mutations in fungi

C - Chemistry – Metallurgy – 12 – Q

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C12Q 1/68 (2006.01) C07K 14/80 (2006.01)

Patent

CA 2436278

This invention relates to disgnostic methods for the detection of one or more cytochrome b mutations in fungi at the position corresponding to Saccharomyces cerevisiae cytochromeb residue 129 that leads to resistance to strobilurin analogues or compounds in the same cross resistance group using any (or a) single nucleotide polymorphism detection technique, preferably using either an allele specific amplification technique such as the amplification refractory mutation system (ARMS) or preferably using an allele selective hybridisation probe technique such as Molecular Beacons or TaqMan. The invention also relates to mutation specific oligonucleotides for use in the method and to diagnostic kits containing these oligonucleotides.

La présente invention concerne des méthodes diagnostiques permettant de détecter une ou plusieurs mutations de cytochrome b dans des champignons, à l'emplacement correspondant au résidu 129 de cytochrome b Saccharomyces cerevisiae, qui conduit à une résistance aux analogues de strobilurine ou à des composés du même groupe de résistance croisée, par mise en oeuvre d'une technique de détection de polymorphisme d'un nucléotide simple, de préférence par mise en oeuvre soit d'une technique d'amplification allèle spécifique, telle qu'une analyse ARMS (amplification refractory mutation system), soit d'une technique de sonde d'hybridation allèle sélective, telle que <= molecular beacons >= ou <= TaqMan >=. La présente invention concerne également des oligonucléotides spécifiques de mutation destinés à être utilisés dans le cadre de ladite méthode, ainsi que des trousses diagnostiques contenant ces oligonucléotides.

LandOfFree

Say what you really think

Search LandOfFree.com for Canadian inventors and patents. Rate them and share your experience with other people.

Rating

Method for the detection of cytochrome b mutations in fungi does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for the detection of cytochrome b mutations in fungi, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for the detection of cytochrome b mutations in fungi will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFCA-PAI-O-2076445

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.