Method for the detection of disease-related prion

G - Physics – 01 – N

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G01N 33/68 (2006.01)

Patent

CA 2563682

Methods for the detection of the disease associated conformation of the prion protein as an indication of transmissible spongiform encephalopathies (TSEs), including preclinical detection of infected live animals and humans, and post- mortem detection methods are disclosed. In one aspect of the invention the tissue or body fluid sample of a test subject is contacted with an antibody that binds only the disease related conformation of the prion protein under non-denaturing conditions.

L'invention concerne des procédés de détection d'une maladie associée à la conformation de la protéine prion en tant qu'indication d'encéphalopathies spongiformes transmissibles (EST), notamment des procédés de détection préclinique d'animaux et d'êtres humains vivants infectés, et des procédés de détection post-mortem. Dans un aspect de l'invention, les échantillons de tissus ou de liquides biologiques d'un sujet à tester sont mis en contact avec un anticorps qui ne se lie à la maladie associée à la conformation de la protéine prion que dans des conditions non dénaturantes.

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