Method for the detection of egfr mutations in blood samples

Details Method for the detection of egfr mutations in blood samples Method for the detection of egfr mutations in blood samples

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2658694

The present invention refers to the detection of EGFR mutations in a blood (serum/plasma) sample from a subject. The method comprises: (i) obtaining the DNA from said sample; (ii) amplifying the nucleic acid sequence corresponding to a specific region of the EGFR gene by means of PCR using a Protein-Nucleic Acid probe; 10 and (iii) detecting said mutation.

La présente invention concerne la détection de mutations du récepteur du facteur de croissance épidermique (EGFR) dans un échantillon de sang périphérique (sérum/plasma) prélevé sur un sujet. Le procédé consiste à : (i) obtenir l'ADN dudit échantillon, (ii) amplifier la séquence d'acides nucléiques correspondant à une région spécifique du gène de EGFR au moyen d'une PCR utilisant une sonde de protéine - acide nucléique, et (iii) détecter ladite mutation.

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