Method for the detection of human hematopoietic short term...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/078 (2010.01) A61K 35/14 (2006.01) C12N 5/00 (2006.01) C12Q 1/02 (2006.01) G01N 33/50 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2428107

Two novel populations of human short term repopulating cells are described. In particular, The inventors have shown that sublethally irradiated NOD/SCID-b2M- /- mice allow the efficient engraftment of two previously undescribed populations of human short term repopulating cells (STRC) that do not produce detectable progeny in the more widely used NOD/SCID mouse. These novel cells are designated short term repopulating cells - myeloid (STRC-M) and short term repopulating cells - lympho-myeloid (STRC-ML) to reflect their different lineage potentials. The invention includes an assay for detecting STRC-M and STRC-ML which is useful in a wide range of applications including assessing of the engraftment potential of human hematopoietic cells, testing the toxicity of drugs on hematopoietic cells and in assessing the viability of hematopoietic cells that have been stored and processed.

L'invention concerne deux nouvelles populations de cellules humaines capables de reconstitution de l'hématopoïèse à court terme. Plus spécifiquement, les inventeurs ont montré que des souris NOD/SCID-.beta.¿2?M?-/-¿ irradiées sublétalement permettent la prise de greffe efficace de deux populations de cellules humaines capables de reconstitution de l'hématopoïèse à court terme (STRC) non décrites auparavant, lesquelles cellules ne produisent pas une descendance détectable dans la souris NOD/SCID, plus largement utilisée. Ces nouvelles cellules sont désignées par les termes cellules capables de reconstitution de l'hématopoïèse à court terme myéloïdes (STRC-M) et cellules capables de reconstitution de l'hématopoïèse à court terme lympho-myéloïdes (STRC-ML) afin de refléter leurs différents potentiels de lignée. L'invention comprend un test pour détecter STRC-M et STRC-ML, utile dans une vaste gamme d'applications, notamment pour tester la capacité de prise de greffe de cellules hématopoïétiques humaines, pour tester la toxicité de médicaments sur des cellules hématopoïétiques et pour évaluer la viabilité de cellules hématopoïétiques ayant été stockées et traitées.

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