Method for the detection of minority genomes in virus...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2407753

The invention relates to a method for the detection of minority genomes in virus quasispecies using DNA microchips. The method makes it possible to detect minority genomes, more particularly minority memory genomes, in a nucleic acids population of a virus quasispecie, which are present in a population of less than 50 %, containing at least one mutation relative to the majority genomes of said quasispecie. Said method involves the following steps: a) extracting the nucleic acid of said virus quasispecie from a sample susceptible of containing said virus quasispecie; b) amplifying at least one fragment of the nucleic acid of said virus quasispecie, and c) detecting and analyzing the existence of minority genomes using DNA microchip-based techniques. The method can be used in genetic diagnosis of viral diseases.

L'invention concerne une méthode permettant de détecter des génomes minoritaires, notamment des génomes mémoire minoritaires, dans une population d'acides nucléiques d'une quasi-espèce virale, ces génomes étant présents dans moins de 50 % d'une population et comprenant au moins une mutation par rapport au génome majoritaire de ladite quasi-espèce. Cette méthode consiste : a) à extraire l'acide nucléique de chaque quasi-espèce virale à partir d'un échantillon susceptible de contenir ladite quasi-espèce virale; b) à amplifier au moins un fragment de l'acide nucléique de ladite espèce virale; et c) à détecter et à analyser l'existence de génomes minoritaires au moyen de techniques basées sur les micropuces à ADN. Cette méthode trouve une application dans le diagnostic génétique de maladies virales.

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